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This study provides a microfluidic-chip-based strategy in which the fluorescent identification of EGFR-expressed cells, in situ cell lysis, MDA and PCR gene amplification are integrated to provide high-quality gene amplification products from which the EGFR multi-mutations information could be acquired using simple and low-cost Sanger's sequencing.
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To allow straightforward validation, our data were acquired using a simple pulse-acquire sequence with a long repetition time, precluding the need to correct for relaxation/saturation effects.
In vivo spectra were acquired using a simple surface coil arrangement with no other source of localization.
These measurements are acquired using a relatively simple non-destructive setup situated in a large hemi-anechoic chamber.
Results are acquired using a standard smartphone camera and analyzed with a simple gray scale quantification procedure.
Protein bands were detected using ECL reagent or ECL plus reagent (Thermo Fisher Scientific Inc. Rockford, 32106, 80197) and images were acquired using the FluorChem E & M imager from Protein Simple (FM0418).
Protein bands were detected using ECL reagent or ECL Plus reagent (Thermo Fisher Scientific, Inc .. Images of immunoblots were acquired using the FluorChem E & M imager from Protein Simple (#FM0418), a digital darkroom technology.
Digital monochromatic images were acquired using ZEN Light Edition Software.
Spectra were acquired using air background correction.
Images were acquired using multipinhole SPECT/CT.
Chromatographic data were acquired using Empower software.
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