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Single-base mismatch detection due to different adsorption propensities of oligonucleotides could be achieved using fluorescence quenching and surface-enhanced Raman scattering (SERS) properties of the dye.
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Calibration of concentrations was achieved using fluorescence correlation spectroscopy inside the cell's cytoplasm [33] (detailed in Methods).
Fluorescence imaging in sections of HCC and nontumoral tissue was achieved using fluorescence photography and microscopy in order to investigate the difference between HCC and nontumoral tissues.
Isolation of intact chloroplasts was achieved using fluorescence-activated cell sorting (FACS) wherein fluorescently stained organelles can be visualized, separated, and collected using flow cytometry.
In fluorescence microscopy of eukaryotic cells, automated single-cell quantification can be achieved using multiple fluorescent probes and channels in a single experiment.
Because adhesions are localized at thin cell edges near the interface with the glass, high signal/noise ratio could be achieved using total internal reflection fluorescence (TIRF) microscopy for time-lapse imaging.
The read out of microarrays can be achieved using a variety of detection techniques [ 3], however, fluorescence is the most commonly used transduction method [ 4].
The development reported to date indicates that unparalleled fluorescence signal reporting in MB-based assays can be achieved using well-constructed Qdot fluorophores.
It was reported that active tumor targeting and spectroscopic hepatoma imaging was achieved using an integrated fluorescence imaging system.
Visualization and quantitation was achieved using a Typhoon 9200 fluorescence Imager (Amersham BioScience, Piscataway, NJ, USA).
Direct readout of applied forces in vivo has been achieved using two related, but distinct, fluorescence techniques that utilise the ability of Förster resonance energy transfer methods to report on changes in distance induced in a biosensor of known mechanical strength.
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