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In this study, we showed that ER, PR, and HER2 status could be accurately confirmed using multiplexed RNA expression profiling, thereby conserving tissue sections and maximizing the value of limited tissue samples from clinical trial patients.
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Model predictions of optimal conditions were accurately confirmed by follow-up experiments.
Equal loading was confirmed using β-actin.
The validity of the models was also confirmed using the Lack of Fit analysis, where an insignificant p value of more than 0.05 was indicative that the model could accurately fit with the actual data [51].
These results were not confirmed using REST.
These observations were confirmed using pharmacologic inhibitors.
This was confirmed using TBR1 null mice.
Convergence was confirmed using AWTY [67].
Protein purity was confirmed using SDS-PAGE.
Functionalization was confirmed using fluorescent GRGDSPC peptides.
Results were confirmed using qPCR analysis.
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