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However, in this project, an important part of data pre-processing and meta-data extraction had to be accomplished by reverse engineering, which indeed is not ideal.
Detection of viral nucleic acid can be accomplished by reverse transcriptase polymerase chain reaction (RT-PCR), real time RT-PCR, or isothermal amplification methods.
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This is accomplished by reverse β-oxidation, which sequentially adds acetyl (C2) units to form butyrate from two molecules of acetate, valerate from acetate and propionate, and caproate from acetate and butyrate (Agler et al. 2012).
The first step in the analysis of a clinical specimen or a viral isolate in our assay is the generation of a DNA copy of the viral RNA, which is accomplished by reverse transcription coupled to PCR (RT-PCR).
Silencing of LHRH-R was accomplished by reverse transfection using the siPORT NeoFX Transfection Reagent and Silencer Select siRNA (Applied Biosystems).
First-strand cDNA synthesis from 1 μg total RNA of C. dichrum was accomplished by reverse transcription using SuperScript III reverse transcriptase (Invitrogen).
Synthesis of cDNA was accomplished by reverse transcription-PCR using an oligo dT) primer and Moloney murine leukemia virus reverse transcriptase (Invitrogen Corporation).
For example, converting PC to DAG (for TAG synthesis) may be accomplished by the reverse action of cholinephosphotransferase or by phosphatidylcholine: diacylglycerol cholinephosphotransferase [ 29- 31].
Marco Rubio said that reversing inequality could be accomplished by turning the earned income tax credit into a subsidy for low-wage earners.
Some could be accomplished by summer.
Hydrolysis can be accomplished by enzymatic, chemical, and hydrothermal methods.
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