This is accomplished by binding of over 400 protein ligands to its various protein interaction-mediating domains and the acetylation of ∼100 protein substrates.
Briefly, nuclear extracts containing NF- κB were prepared using a Nuclear Extract kit (Active Motif, Carlsbad, CA), and the capture was accomplished by binding to a consensus oligonucleotide (5′-GGGACTTTCC-3′) immobilized on a 96-well plate (Millipore Corp).
Micelle stabilization was accomplished by binding of model drug (doxorubicin) via an acid-sensitive hydrazone bond and incorporating one to four 11-aminoundecanoic acid (AUA) moieties to manipulate the hydrophobic/hydrophilic ratio.
The first step in the recognition of chemical signals (peripheral events) is accomplished by binding and membrane receptor proteins that recognize external ligands and translate this interaction into an electrical signal to the central nervous system.
Pairing may be accomplished by proteins binding to identical sites in linked copies, which subsequently form homodimers that strengthen chromatin association [36], [38], [39], [46].
This targeting could be accomplished by specific RNA binding proteins and/or miRNAs binding to the 3′ UTRs of hid and reaper mRNAs and directing them to the 5′-3′ degradation machinery (Eulalio et al., 2007; Jones et al., 2012; Nishihara et al., 2013).
Augmenting the model with a negative feedback through OCT4, as suggested by [ 5, 9], can be accomplished by increasing the binding affinity of the OCT4 protein to the Nanog regulatory site, and decreasing the stability of the OCT4-containing RNAP II complex.
An alternate method of derivation may be accomplished by incorporating a mercury-binding site into RNA via a phosphorothioate in the RNA backbone.
Gene product imaging can be accomplished by measuring the accumulation or binding of the respective radiolabeled substrates or ligands.
Learning RBP binding motifs can be accomplished by applying DNA motif finders, which only consider the RNA sequence, or by considering RNA secondary structure either explicitly or as a layer on top of sequence preference.
