Infection by the SFTSV pseudotypes could be abolished using sera from convalescent SFTS patients.
A number of studies have demonstrated that the protection offered by RIPC to the heart and other organs can be abolished using inhibitors of various opioid and cannabinoid receptors [ 78– 83].
When RME-8 expression was abolished using RNAi, the morphology of endosomes was profoundly altered becoming a highly branched network of membrane tubules that contained many membrane proteins that require retromer and the WASH complex for their proper localization including the iron transporter protein SLC11a2 (also known as DMT1-II) and the α5β1 integrin complex.
However, this ability of E2 and 25HC was abolished using 10µM ICI (Fig. 7A B and Fig. S4), suggesting that the action of both compounds involves an ER-mediated mechanism.
Next, the hypoxia-induced expression of HIF-1α and CTGF was abolished using the inhibitor of DNA-primed RNA synthesis actinomycin D (Fig. S3), suggesting that transcriptional mechanisms are responsible for the up-regulation of both HIF-1α and CTGF.
Furthermore, the spatial rearrangement of GABAARs could be abolished by using gabazine, a specific antagonist of GABAARs, or by depolymerizing MTs using nocodazole [24].
Further investigation showed that this FRET change occurred only with a linear DNA substrate and was abolished by using a multiply mutated Pol I KF) in which binding to the 3′ 5′ exonuclease site is severely compromised.
In addition, syringaresinol, the hydrolysate of liriodendrin, more potently down-regulated production of pro-inflammatory cytokines and suppressed NF-κB and Akt signalling pathways in LPS-induced RAW 264.7 macrophages,which were abolished by using a pure ER antagonist, ICI182, 780.
The HIS6-tagged virions bound IMAC matrix beads in a pH dependent manner (Fig. S2), and the binding could be specifically abolished using imidazole (Fig. 1D).
These positive signals are significant, as the immunoreactivity could not be abolished by using E. coli protein lysates as competitors.
