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Accordingly, long MLCK may be a third protein substrate of ARD1 in mammals.
We agree that there may be a third protein or RNA in the complex.
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More importantly, we found FANCD2 can regulate translesion synthesis (TLS) DNA polymerase eta (Fig. 3, 4), which appears to be a first protein to be indentified acting as a mediator of FANCD2 function.
Further work by Pierrel and co-workers aimed to answer two important questions: does MiaB function as a radical SAM enzyme, and is MiaB sufficient for modification of iA to msiA or is a third protein required?
These are a first protein fused to a DNA-binding domain of known specificity (hybrid 1); a second protein fused to a transcriptional activation domain (hybrid 2), that can interact with the first protein, constituting a functional, albeit composite, transcription factor; and one or more reporter genes transcribed based on the binding of the composite transcription factor.
Analysis of the completed genome showed that there was a second protein with homology to BamB encoded outside of the aromatics island of the genome.
γ-SNAP is a third SNAP protein widely expressed in mammalian tissues but is less similar to Drosophila SNAP and α-SNAP than β-SNAP.
RanBPM is a third interacting protein, that is expressed in several cell types, is induced in PHA stimulated peripheral blood leucocytes, and when overexpressed as a truncated protein can affect microtubule function.
This was done by introducing a third protein into the system in the yeast three-hybrid assay.
First, there is a third two-domain protein (SorbiEULD1C) for which no ortholog could be identified in rice.
DAP5/NAT1/p97 is a third eIF4G-like protein, strongly related to the C-terminal two thirds of eIF4G but entirely lacking the N-terminal third.
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