Although NANOG is dispensable in some instances of iPS reprogramming, it is a stringent selection marker for germline-competent iPS cells with increased ES cell-like properties [ 20].
This was a stringent selection criterion, given that knockdown of receptors for two of the four defined medium components (insulin, EGF, transferrin and isoproterenol) did not achieve this level of growth inhibition.
Therefore, it is vital that a stringent selection process to eliminate any undifferentiated ESC before applying into human trials.
17, 18 To select this novel SERM, a stringent selection process was implemented to assess its biological activity and tissue selectivity on key endpoints, which included the uterus, lipid metabolism, bone remodeling, and central nervous system function.
Gblocks server was applied with the 'codons', 'DNA' and 'protein' mode respectively for PCG nucleotide sequences, rRNA sequences and PCG amino acid sequences, and with all options for a stringent selection were chosen.
A stringent selection process was used to define a list of 105 genes that are significantly (p ≤0.001) over expressed in body parts from the resistant versus susceptible strain.
The cut-off for selecting primary hits was set as fold change > mean + 3 S.D. relative to DMSO control, which is a rather stringent selection criteria based on previous studies (Borowiak et al., 2009).
A stringent selection process is also vital to maintaining standards, Smith says.
All array probes were designed from known transcripts and ESTs based on the chicken genome assembly WASHUC1 (Dec. 2004), and a stringent selection of probes was performed before the analysis.
We used a stringent selection criterion, that is, qualified only alleles demonstrating an absolute binding score difference of 2.0 or more.
