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Hybridization of clone 9f to RNA separated by electrophoresis revealed two species 700 and 1100 nucleotides long but genomic blotting indicates that PV may be a single copy gene.
The following recommended criteria were used to establish a comprehensive gene set; the gene should (i) be long enough to contain phylogenetically useful information, (ii) not be subject to horizontal gene transfer, (iii) be a single copy (iv) have at least two regions sufficiently conserved that allow the design of amplification and sequencing primers and (v) predict whole-genome relationships.
Therefore, fragments of ConanN2_AC were assigned to be a single copy when they were separated by less than 100 bp.
This number is based on comparison to SRY, which until recently was believed to be a single copy gene in suids (see [ 1]).
Physical localization of TaSTOP1 by Tyr-FISH technique not only confirmed the results obtained from PCR based mapping, but also indicated that TaSTOP1 could be a single copy gene localized on the long arm of homoeologous group 3 chromosomes.
M. genitalium strains resistant to azithromycin contain resistance mutations in region V of 23S rRNA, which is encoded be a single copy gene in M. genitalium [ 1, 10, 13, 14, 18].
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Data from genomic Southern blotting were consistent with mouse Treh being a single copy gene.
DNA gel blot analysis showed that TrPI is a single copy gene in the T. rupestris genome.
In 37 out of 43 strains there was a single copy of the pspC gene, while two tandem copies of pspC were found in the other six strains.
Stoichiometry experiments revealed that there is a single copy of GCP5 and multiple copies of gamma-tubulin, GCP2, GCP3, and GCP4 within the gamma-tubulin complex.
Genomic analysis suggests that it is a single copy gene in L. donovani, and its homologues are present in members representing other Leishmania species complexes.
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