Note that because time scaling (i.e. differentiation efficiency) is a replicate-specific random effect we need to use the same samples for qRT-PCR validation.
issue was fitted, where y = ln(1 + count e ), μ was mean, tissue was the tissue effect, replicate was the tissue replicate effect, gene was gene effect, exon number was exon effect where exon's are ordered as a fraction of the total exon number and count e was the read count per exon.
Genotype, replicate, and error were considered random effects, whereas density was considered to be a fixed effect.
Replicate was a random effect with repeated measures.
The number of embryos at 14 days gestation did not differ between females from the monogamous (mean = 7.91 ± 0.29) and polygamous lines (mean = 7.59 ± 0.36) (F 1, 6 = 0.22, P = 0.655); although there was a significant effect of replicate line nested within selection history (F 6, 56 = 2.35, P = 0.042) (whole model: F 7, 56 = 2.10, P = 0.059).
αtri is a random effect for technical replicate i within each biological replicate r, which is assumed to be uncorrelated with mean zero and variance σ2α.
Replicate pool was a random effect in the male models.
Importantly, although the lack of category-level guidance is a null effect, it was replicated across three separate experiments (noting that the positive finding in Experiment 1 could be explained by visual similarity) with separate groups of participants, and was in contrast to the consistent and robust guidance that we found when asking participants to encode the visual image.
The authors state that there was a significant effect of the replicate on Principal Component 2 (page 3, left column, third line from bottom), but later state that " sub-strain differences were comparable in the two replicates conducted one year apart" (page 5, left column, line 5 from top).
Importantly, at both high and low male densities, males who nested had greater red throat indices (Fig. 1b).> -wrap-foot> Repoolate pool was a random effect in all models and significant results are indicated in bold Male density was an important predictor of the number of nesting males.
