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TargetScan analysis revealed miR-206 to be a putative miR that potentially targets the 3′UTR of LXRα mRNA.
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It was found that there is a putative miR-21 binding site at the 3′UTR of TPM1 variants.
DOI: http://dx.doi.org/10.7554/eLife.01323.028 In the miR-1 null animals, myosin light chain kinase (MLCK) was a putative miR-1 target of particular interest, as it was highly upregulated and had a known role in regulating the cytoskeleton.
Using bioinformatics analysis, RB1 gene was determined to be a putative target of miR-192 and luciferase reporter assays confirmed direct binding of miR-192 to the 3′-UTR of this gene [ 76].
These findings suggest that miR-194 might be a putative target molecule for regulating metabolic and bone disease.
Furthermore, we showed that miR-503 was able to reduce S phase cell populations and caused cell growth inhibition, suggesting that miR-503 may be a putative tumor suppressor.
In consideration of μG as a potential stimulant of oxidative stress primarily induced by depletion of the energy [ 51], miR-200a could be a putative signature of the host response to LPS assault mediated by μG.
Both indicated that a conserved sequence in the 3′-UTR region of dMyc's transcript is a putative target of miR-308 and other members of the miR-2 microRNA family (Fig. 3A).
As PTEN is a putative target for miR-205, we next located potential binding sites of miR-205 in the PTEN 3′-UTR region.
We focussed on transgelin 2 (TAGLN2), which was one of the most downregulated genes in oligo-microarray studies using an miR-1 transfectant and is a putative target gene of miR-1 and miR-133a as suggested by web-based software.
Further analysis of possible mechanisms revealed that the Bmi-1 oncogene was a putative target of miR-128a.
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