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For no less than 90% specificity to recognize the positive candidate in this uricase pair, the mean of specific activity plus 1.4-fold of the SD of the starting material can be a practical threshold (Additional file 1: sheet 2: ROC analysis of activity).
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There may be a practical payoff, too.
I claim to be a practical idealist.
In the absence of any published definition of prolonged LOS in Europe, the cut-point defining the upper quartile of patients (>20 days) was selected as a practical threshold for prolonged LOS; this also happened to be 1 week longer than the median LOS (13 days).
This helped to find more points whose peak intensities were otherwise below a practical threshold (Figure 2F).
The existence of mechanisms leading to a dose response that is non-linear and has a practical threshold is increasingly recognized, not only for compounds that interact with non-DNA targets but also for DNA-reactive compounds [10].
By the comparison of specific activity to recognize a positive candidate, a practical threshold is required.
"It's a practical thing.
This was a practical necessity.
"It was a practical reality".
There is a practical link, too.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com