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MS/MS of unreduced, trypsin digested and iodoacetamide labeled sample also confirmed Cys406 to be a peptide containing free cysteine (Figure 8C).
An interesting example could be a peptide containing a pair of acidic amino acids and coordinating a magnesium ion as seen in a great variety of unrelated modern nucleic acid metabolism enzymes (polymerases, nucleases, ligases, topoisomerases, and more) [ 133- 135].
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Substrate for BoNTA was a peptide containing residues 187 203 of SNAP-25 [37], while that for BoNTB was residues 60 94 of VAMP [51].
It belongs to the G-protein-coupled receptor superfamily and is a peptide containing 360 amino acids.
The substrate utilized is a peptide containing amino acids 379 382 of human p53 (Arg-His-Lys-Lys[Ac]), an established target of Sirt1 activity; Sirt1 activity is directly proportional to the degree of deacetylation of Lys-382.
Using an in vitro assay we show that fOSGN2-P generation is reduced when permeabilised cells are incubated with a peptide containing the N-glycosylation consensus sequence.
A spatiotemporal signature similar to H3K36ac was also observed when we used antibodies targeted to H3K27me3, which were raised using a peptide containing the motif ARKme3SAP.
SAPNs are formed from a peptide containing a pentameric and a trimeric coiled-coil domain.
Block copolymer brushes with defined thickness comprising a non-fouling POEGMA or PAAm block and a short terminal PAA block were functionalized with a peptide containing the arginine-glycine-aspartic acid (RGD) sequence.
The antibody used to immunoprecipitate PPP1R2-related proteins was raised against a peptide containing amino acid residues 134 147 from the mouse PPP1R2 sequence.
For the wild type enzyme phosphorylation was also present on a peptide containing Ser552 and Ser554.
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