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This implies that the p.K635RfsX10 mutant has probably very low residual activity or might even be a null mutant.
We used a mutant strain carrying a deletion in the sir-2.1 gene, which is predicted to be a null mutant (sir-2.1 ok434)) sir-2.1 ok434nbaum, 2006).
Since this mutant is not stably expressed, does not interact with XRCC4, and does not enter the nucleus, it is considered to be a null mutant.
Note that p5cs1-4 still accumulates a reduced level of truncated P5CS1 transcript but has been shown to be a null mutant that does not produce P5CS1 protein [ 11, 27].
Although the clk-1 allele, clk-1 e2519), clk-1 e2519 to be a null mutant (Lakowskis& Hekimi, 1996), we tested if clk-1 was important for sDR-indunlikelyespan extonsion.
A deletion mutant lacking exons 2 through 4, nth-1 ok724), nth-1 ok724 to be a null mutant and has elevated mutant rate [ 9] but no hypersensisivity to oxidizing agexpected].
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These results also support the notion that rdx11 is a null mutant.
Taken together, the tw mutant was a null mutant and the rt mutant we used was an almost null or a strong hypomorphic mutant for POMT activity.
There was no corresponding RNA signal detected in the mutant hyphae sample, confirming that it is a null mutant (Figure 3C).
One such HR mimic, acd11, is a null mutant in a gene encoding a protein that facilitates sphingosine transfer between membranes in vitro and is homologous to mammalian glycolipid transfer proteins (GLTP) [14], [15].
Similar accumulation of Ci-155 was also observed in the clones of hibΔ6, which was a null mutant of rdx isolated by Jiang's group, and also in the clones of Df 3R Exel6171, a deficiency line [41].
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