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This motif has been recently shown to be a functional sequence that is used to target the ribosome binding sites of target mRNAs such as RNAIII [84] and RsaE [26].
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Therefore, it is possible that ACTGAC could be a functional branch-point sequence for the splicing of T. vaginalis introns.
For example, assuming that T j controls T i, there may be a functional polymorphism in gene j's sequence, but no observed variation in expression level, T j.
To examine if 14-3-3 14-3-3 14-3-3ctional 14-3-3 sequence, we examined if it could complement the rapamycin sensitivity of mutants lacking bmh1 or bmh2.
Thus the fusion does not interfere with the operation of M.EcoKI and the enzyme is still a functional sequence-specific MTase.
Selective constraint is defined as the factor by which evolutionary divergence of a functional sequence is reduced due to the action of purifying selection [ 8].
Selective constraint (also known as functional or evolutionary constraint) is defined here as the factor by which evolutionary divergence of a functional sequence is reduced, relative to a neutrally evolving sequence, due to the action of purifying selection [ 10].
This polymorphism may nevertheless be in linkage disequilibrium with a functional sequence change as recently demonstrated with the identification of the rs10490924 polymorphism in the ARMS2 gene which results in instability of the transcript [62].
Subsequent DNA synthesis will replicate the plasmid (and the gene), which can potentially restore vitality to the strain (permitting survival without supplemental thymidine) should a functional sequence be made.
The thresholds of the bidirectional BLAST (BLASTp) for a functional sequence were set to have an e-value less than 1 × 10-30, aminoino acid sequence identity above 40%, and a matching length at least 70% of the query sequence.
Taken together, despite >90% sequence identity between wt- and neoPTE, the reverse trajectory led to a functional sequence that is poorly connected with the original one.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com