The interaction type in STRING is a functional association, which the authors define as "the specific and meaningful interaction between two proteins that jointly contribute to the same functional process".
To test if there was a functional association of HoxA-11 and FOXO1A we cotransfected HeLa cells with a luciferase reporter construct containing the dPRL enhancer dPRL-332/luc3 dPRL-332/luc3ion vectors for Hoxand1, FOXO1A or both (Fig. 4).
STRING is a functional association network that contains associations inferred from various data sources (experimentally verified interaction, co-occurences in the literature, coexpression, and similar genomic context).
The second one is a functional association network (FAN) consisting of 1,217,908 interactions and 16,648 genes downloaded from the STRING database [ 35].
A region spanning residues 403 504 of DISC1 was later shown to be a functional self-association domain by pull-down experiments with deletion mutants expressed in HEK293 cells.
Each gene/process pair was assigned a functional association score equal to the ratio of its average probability of functional relationship to the process over the process's cohesiveness: (8) This calculation was also used to predict each biological process's predicted association enrichment with unknown genes.
In vitro mutagenesis studies indicated that p.R215W might be a functional mutation that impairs the association of NBN with γH2AX [ 21].
The co-expression networks are consistent with a functional association connecting CQ, pfcrt and hemoglobin digestion, and also reveal novel associations that will need future validations.
This, although not a bone fide caveolin-binding motif, has been shown to be critical in mediating a functional association between caveolin-1 and G-protein-coupled receptor kinases (Carman et al, 1999).
The high Xd-score for this gene set pair (0.80, the significance threshold is 0.45) points to a functional association via multiple connecting molecular interactions, which is confirmed by the visualization.
