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This labeling of rod terminals is unlikely to be a fixation artifact, since the sections were lightly fixed and labeling was specific to synaptic terminals.
Therefore, this substitution was considered to be a fixation artifact and the case was classified as wildtype.
Another possible explanation for the cytoplasmic location of HMGB1 could be a fixation artifact with redistribution of nuclear HMGB1 to the cytoplasm.
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The previous observation of GFP-Polo filaments in live oocytes also ruled out the possibility that the failure to find filaments was a fixation artifact, and indicated that the cause of the failure to find the filaments using our standard protocol for live-imaging reflected a way the experiment was methodologically different from previous GFP imaging experiments.
TEM further depicts that morphology and orientation of cellular protrusions looks fully intact indicating that the interstitial space including filigree protrusions of mesenchymal stem/progenitor cells appears real and is not caused by a fixation artifact.
Despite these caveats, if a fixation artifact is indeed concealing the fact that mammalian MCM-Cdc45 is localized at forks during DNA replication, then it becomes much easier to reconcile these findings.
It's a fixation.
This might be a sample artifact.
It is important to note that the cup-shape appearance of exosomes visualized by electron microscopy may be an artifact of the fixation process, and their true shape mirrors that of round microvesicles [ 17].
This low incidence of blebbing in the untreated bacteria suggests that the observed changes in treated bacteria were not fixation artifacts.
However, it is also stated that indentations could be an artifact caused by the fixation [12].
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CEO of Professional Science Editing for Scientists @ prosciediting.com