In summary, data presented here strongly suggest that locally generated ATP is an important regulator for actin-based cytoskeletal dynamics involved in cell extension and motility and that CK-B is a controlling enzyme in the compartmentalization of ATP availability.
Hydroxymethylglutaryl-CoA synthase is a control enzyme in the synthesis of ketone bodies, and is highly expressed in liver and colon, and low in testis, heart, skeletal muscle and kidney [ 35].
For example, a Controller could be an enzyme, controlling a reaction, which is used as Controlled object.
Phosphorylation is a common mechanism for controlling enzyme activity, and involves the addition of phosphate groups to specific amino acids by enzymes called kinases.
The position of the substrate (GSTP), product (GST), and the conspicuous GST signal emanating from reactions in which high concentrations of GST were introduced as a control enzyme (lanes 1 and 4) generating a signal that overlaps with the GST signal is indicated.
Pase (matched for protein content in PB 0.1 M, Sigma) was used as a control enzyme.
HMGS catalyzes the condensation of Acetyl-CoA with acetoacetyl-CoA into HMG-CoA and HMGR is a rate-controlling enzyme that catalyzes the reduction of HMG-CoA to mevalonic acid (MVA).
Phosphofructokinase (PFK) is a rate-limiting, allosterically controlled enzyme that catalyzes the conversion of fructose-6-phosphate to fructose-1,6-bisphosphate (F1,6BP) before its conversion to 3-carbon molecules and ultimately to pyruvate.
Dried blood spot (DBS) acid α-glucosidase testing, with neutral α-glucosidase as a control enzyme, was measured using a previously described fluorimetric method [ 1, 2].
Dicer1 is a critical enzyme controlling the synthesis of miRNAs.
