In this process, an Nd YAG laser beam was focused near the glass target while the target was inserted in a chamber containing SF6 atmosphere or at vacuum condition.
After cutting, slices were transferred to a chamber containing ACSF at 37°C, in which 50% of the NaCl was replaced with choline chloride.
Samples of the reaction mixtures, containing 20 μg of xylose-equivalent sugars, were applied to a TLC sheet (Silica gel 60 F254, Merck, Darmstadt, Germany) that was placed in a chamber containing 7 1 2 solvent mixture of n-propanol:ethanol:H2O.
All slices used in optical recording were stained with a voltage-sensitive dye (RH155 0.2 mg/ml, Nippon Kanko Shikiso Kenkyujo, Okayama, Japan) for 15 min, and were kept in a chamber containing oxygenated ACSF for at least 30 min for recovery as described previously.
Dams were anesthetized in a chamber containing 2.5% isoflurane in oxygen and euthanized by cervical dislocation at indicated stages and embryos were collected in phosphate-buffered saline (PBS), then staged and fixed by immersion in ice-cold fixative (2% paraformaldehyde/0.2% glutaraldehyde) for 5 to 15 minutes (depending upon their developmental stage).
The plate was placed in a chamber containing a solvent system of acetic acid-N-butanol-water (1 2 1) for a while and then was withdrawn.
Prior to each experiment, the animals were placed in a chamber containing 2% isoflurane in oxygen.
Rat pups and their dams were placed in a chamber containing 20 ppm NO during the first week of life.
Nematodes are first immobilized in either the anesthetics levamisole or sodium azide, then transferred to halocarbon oil and finally mounted in a thin glass capillary, which is immersed in a chamber containing refractive index-matching fluid (see Materials and Methods).
Prior to each experiment, the animals were placed in a chamber containing 2.5%% isoflurane in oxygen.
