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One row of a 96-well plate was added culture medium only to be a blank control.
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The 12th well in every row was a blank control.
Human hepatocellular carcinoma tissue RNA was a positive control and water was a blank control.
The cells was treated as follows: group A, blank controls; group B, transfected with 100 pmol scrambled siRNA/SilenceMag; group C, transfected with 100 pmol hVEGF siRNA/SilenceMag; and group D, transfected with 100 pmol I-hVEGF siRNA/SilenceMag.
However, all those conclusions were ambiguous because a blank control was lacking in those studies.
Distilled or deionized water was added to the plates to be used as a blank control.
Substrate only was used as a blank control, whereas worms were boiled before homogenization for negative controls.
Culture medium was used as a blank control and luminescence was measured using an MLX microtiter luminometer (Dynex Technologies Inc., Chantilly, VA).
One group of these cells was subsequently treated with 87.8 μM oridonin (Gracia Chemical Technology Company, LTD, 98% purity, HPLC) dissolved in DMSO (final DMSO concentration in growth media is 0.1%), and another was used as a blank control group cultured in medium containing 0.1% DMSO for 24 hours.
The pre-MACS suspension was further divided into two equal parts, one of which was used for a blank control.
Cleavage of the tetrapeptide substrate in the absence of CypA was used as a blank control, and the addition of CypA was used as a positive control for PPIase activity.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com