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To evaluate how well a simulated Bcd profile in a given region of the embryo fits an exponential function, we also calculate Adjusted R-square (Adjusted R2) values [41].
Live imaging experiments that simultaneously monitor Bcd and gap gene products in embryos exposed to the temperature step would answer three important questions: when gap domains are established, if gap domains are refined through later cross-repression or come up in the proper position despite and abnormal Bcd profile, and if the variability of gap domains is increased in these embryos.
Embryos exposed to a uniform temperature of 23°C in a microfluidic device displayed a normal Bcd profile that was precise over nuclear division cycles 11 13 (Figure 3A C, Movie S5), as previously reported [8] for embryos developing under standard conditions outside of a microfluidic device.
This Bcd profile was excluded from the further analysis.
Bcd profile #54 provides the only exception from this basin-to-basin transition rule.
For the median Bcd profile, the gap gene expression patterns generated by Eqs.
Similar(37)
Because different Bcd profiles lie on different portions of the Bcd-Cad plane, we first characterized which combinations of attractors are present in different parts of this plane by performing a bifurcational analysis of the shorted model (2).
Unlike correction of abnormal nuclear densities seen in histone-eGFP embryos, embryos with abnormal Bcd profiles did not always correct for abnormalities in the Bcd gradient by cycle 14.
The length constant λ of the nuclear Bcd profiles at nuclear cycle 14 in these simulations is 145 µm; while this value is somewhat higher than experimental measurements, those at earlier nuclear cycles are smaller and closer to experimental values (107, 124, 134 and 141 µm at nuclear cycles 10 13, respectively).
We studied the model on the newly normalized Bcd profiles in order to crosscheck our results.
Roughly speaking, we can associate the alternatively normalized Bcd profiles with Family I.
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