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To specifically evaluate our model on this critical feature, we simulated Bcd gradient formation and analyzed Bcd concentration profiles at distinct nuclear cycles.
We plotted two separate profiles: local DNA-bound Bcd concentration [Bbound] and nuclear Bcd concentration [Bn], which are shown in Fig. 2A and B, respectively.
In these figures, Bcd concentration profiles at nuclear cycles 10 14 are shown.
Fig. 1A represents a simulated 2-D embryo at nuclear cycle 14 showing the local total Bcd concentration [Btot].
Unlike the stable nuclear Bcd concentration profile, target gene expression patterns evolve significantly as a function of developmental time.
Recent studies show that the nuclear Bcd concentration profile is established as early as nuclear cycle 10 and it remains stable until nuclear cycle 14 [33].
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In our simulation results, nuclear Bcd concentrations [Bn] are calculated from the local DNA-bound Bcd concentrations [Bbound].
Our results reveal an association between transcriptional burst events and Bcd concentrations on all three scales.
One of the most striking features of the Bcd gradient dynamics is the stability of nuclear Bcd concentrations during nuclear cycles 10 14 [24].
Consistent with experimental findings [24], our simulated results show that the profiles of nuclear Bcd concentrations [Bn] are stable during nuclear cycles 10 14 (Fig. 2B).
In addition to the hb intron dot data extracted from our Confocal images, we also measured simultaneously Bcd concentrations in embryos.
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