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Table 2 gives the BBI scores in the three surveys divided into predefined risk groups.
There was a statistically significant time-related deterioration of BBI score in the whole group of participants (p < 0.001, betta 12.5 BBI scores/year [CI 8.0; 17.0], adjusted R square 0.25) and in all subgroups except for the auxiliary nurses with work experience from medical departments.
The simultaneous and fast determination of BBI and lectin in soybean is proposed, for the first time, in this work.
This fact has been related to the presence of Bowman-Birk inhibitor (BBI) and lectin in soybean.
The extracts obtained were analysed by RP-HPLC ESI-MS foRP-HPLC ESI-MSidentification of BBI and lectin in soybean.
Moreover, a fast chromatographic methodology using a perfusion column and UV detection was optimized for the rapid determination of BBI and lectin in soybean.
After evaluating its analytical characteristics (linearity, precision, and recovery), the method was applied to the quantitation of BBI and lectin in different soybean varieties.
Although VP2 cleavage by 1 µg C. sonorensis saliva proteins, or the higher concentrations of chymotrypsin (0.05 µg/ 50 µl) was only partially inhibited under these conditions, in both cases it was completely prevented by addition of 20 µg of BBI (Examples shown in Figure 7A).
MY, MV, OA, RF, AD and BBI took part in the data collection.
