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Lowering the ionic strength of the bathing solution enhanced potentiation, suggesting that it involves electrostatic interactions.
Samples of bathing solution were collected 10 minutes after the concentration of oxytocin was maximal.
It is known that the water content of specimens in this method depends on the osmolality of the bathing solution.
It was observed that the bathing solution had significant influence on the tensile behavior of the corneal samples.
This was established experimentally by showing that removal of calcium from the bathing solution results in marked reduction in transepithelial resistance and coincident increase in paracellular permeability.
Manipulation of the cartilage physical environment was achieved through (1) removal of the superficial tangential zone (STZ) and (2) varying the saline bathing solution concentration.
Corneal strips were first air-dried and then soaked in a bathing solution until they reached an average thickness ranging from 0.3 mm to 1.1 mm.
The bathing solution was maintained at 37 ± 0.5 °C and was bubbled with 95%% O2 and 5%% CO2 (pH 7.4) throughout the experiments.
To exclude this possibility, we eliminated membrane potential using high potassium bathing solution (133 mM KCl).
Using ex vivo corneas allows for easier pharmacological manipulation and ion substitution of the bathing solution.
This gap allowed the cells at the lower surface access to the bathing solution.
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