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A dye bath was prepared at room temperature by adding bleached cotton fabric and required amount of Glauber's salt.
A dye bath was prepared at room temperature using 1 g/L wetting agent, 5 g/L NaOH, 3 mL/L H2O2, 50 % w/v (i.e. 50 g of hydrogen peroxide was weighed and was diluted up to 100 ml of water). 1 g/L sodium silicate (peroxide stabilizer).
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The electroless deposition bath was prepared according to recent study [23].
The bath is prepared by adding the constituents in appropriate sequence.
ETV was prepared at BMS.
Nickel coatings were prepared at different concentrations of NH4Cl as a crystal modifier in the electrodeposition bath.
Be prepared at all times.
A solution of GR113808 (a 5-HT4 receptor antagonist) was prepared in DMSO at a concentration of 1 mg/ml and 50 μl was added to the organ bath for a final concentration of 2 μg/ml (n=6).
Atropine (an acetylcholine receptor antagonist) was prepared in physiological saline at concentration of 100 μg/ml and 250 μl was added to the organ bath for a final concentration of 1 μg/ml (n=4).
10 mL mixture of H2SO4 98% and HNO3 70% (ratio 7 3) was prepared in ice bath.
A filtered 3% (w/v) FFA-free BSA solution was prepared with ddH2O and maintained at 55°C in a water bath.
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