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The content of NiCl2·6H2O in the plating bath was fixed at 1 mol L−1, while the content of CoCl2·6H2O was varied in the range of 0 0.05 mol L−1.
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The deposition temperature was fixed at 70 °C by an oil bath.
Judgment was fixed at $12 million.
The Sp value was fixed at 80%.
The pressure was fixed at 1 atm.
Fish density was fixed at 0.1 fish·m−2.
Minimal statistical significance was fixed at p<0.05.
The temperature was fixed at 30°C.
The preparations were fixed at the bottom of a 2.5-ml perfusion organ bath by two small needles and continuously superfused with oxygenated Tyrode's solution at a rate of 2.7 ml min−1 together with solutions of the reference compounds or solvent at a rate of 300 μl min−1.
After the third cycle, they were fixed at 1 g [ 8] and maintained in an organ bath containing aerated Krebs solution (95% of O2, 5% of CO2), which was changed every 15 minutes for 50 min to allow stress relaxation.
The energy is fixed at crescendo level.
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