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Six laboratory batches were generated by the method described above.
Spore batches were generated by cultivation on soy flour-mannitol (SFM) agar plates [ 13], harvesting by scraping of spores and suspension in 20% (v/v) glycerol, and storage in aliquots at −80°C.
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The batch files for constrained searches were generated by TreeRot 3 [ 95].
The first three profiles were generated by RNAseq from three batches of embryos in 3-fold, from which 32%, 355 %and35%5 % of yolk was removed.
Most opportunities, though, were generated by individuals.
All embryos were generated by natural mating.
Mononucleosomes were generated by salt dialysis [81].
Peptides were generated by Sigma-Genosys.
M6 mutant alleles were generated by excision.
Embryos were generated by natural crosses.
Transgenic mice were generated by pronuclear injection.
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