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This result and all subsequent Western blot analyses presented have been confirmed on at least three batches of tissues.
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Seven batches of tissue samples were subjected to 2-DE.
The statistical analysis of the volumes (% volume) of these spots from seven batches of tissue samples is shown in Figure 2B.
To ensure reliability and reproducibility, seven batches of tissue samples were subjected to proteomic analysis using ImageMaster 2D Platinum Version 5.0 software and fourteen gels were generated.
Leaves from five virus-infected grapevines along with their respective controls were used separately and pigments estimated by two independent times using separate batches of tissue.
As such, an added benefit of having access to a long-term culture model that stably maintains a consistent phenotype and genotype over prolonged periods is that multiple studies or multiple repeat doses can be performed with a single batch of tissues or cells.
Decay-corrected counts-per-minute data of the daily batch of tissue samples (≈100 for 2 compounds) were measured on a γ-counter LKB Wallac 1282 Compugamma within 2 h.
Each batch of tissue samples required a minimum run time of 3 days from tissue processing to result.
These researchers have rejected the more traditional path of cell therapy that you may have read about over the past decade of hope around stem cells – the idea that stem cells could be used to create batches of functioning tissue (heart or brain or whatever else) for transplant into the damaged part of the body.
Batches of 10 14 tissue fragments were successively dissociated by means of a Pasteur pipette in a PBS solution with 5.5 mM glucose (Sigma), 100 U/ml penicillin and 100 µg/ml streptomycin (both from Gibco), with no enzymatic digestion.
To prevent variable results yield by different batches of materials, tissue sections used in this study were stored in cryoprotectant (0.05 M phosphate buffer, 30%% ethylene glycol, 20%% glycerol; at −20 °C) such that sections of different sections of different age groups were processed at the same time.
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