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Multiple print batches of slides were used, with 41 samples hybridized in replicates on different batches.
* Hands-on time and total time were estimated based on 3 batches of slides prepared 8-100 slides per batch).
Different case types were embedded in batches as they came from the tissue processor, then moved in batches to the microtomy stations where batches of slides were prepared, and the sections made.
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If less than 80% concordance was achieved between evaluations, the entire batch of slides was re-read.
Positive and negative controls were performed with each batch of slides.
A 32-pin spotting configuration was used to print a batch of slides centrally within the slide's active surface.
A positive control consisting of a formalin-fixed paraffin-embedded tissue section of melanoma demonstrating reactivity for the cocktail was processed with each batch of slides.
A likely reason for these less significant p-values is that the protein and lysate production for this batch of slides was not of the same quality as the protein and lysate production of the previous experiments.
Nowadays, sophisticated Virtual Microscope systems can be acquired, with the capability to quickly scan large batches of glass slides at high magnification and compress and store the large images on disc, which subsequently can be consulted through the Internet.
On the one hand such an algorithm allows the automatic evaluation of large batches of immunohistological slides in an automatic high-throughput manner.
Furthermore, 12 samples were assayed twice on separate dates (four months apart and with different inner PCR reactions, batches of microarray slides, enzymes and reagents).
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CEO of Professional Science Editing for Scientists @ prosciediting.com