OSCC tissue sections with known immunopositivity for 14-3-3ζ 14-3-3ζ 14-3-3ζrted earlier [ 2], were used as proteine control in eash batch of sections analyzed.
Positive and negative controls were included with each batch of sections to confirm the consistency of the analysis in all of the stains that were performed in this study.
For every batch of tumor sections, appropriate quality control sections were used for FISH testing.
Commercially available antibodies were used for the immunohistochemistry, with a control section for every batch of 20 sections, and evaluated blinded by a senior pathologist (TS).
Sections from embedded MCF-7 cell pellets (described above) were included as controls for each batch of tumor sections.
Two batches of unstained melanoma sections were sent, in May and November 2012, to the 46 laboratories supported by the French National Institute of Cancer (INCa).
To prevent variable results yield by different batches of materials, tissue sections used in this study were stored in cryoprotectant (0.05 M phosphate buffer, 30%% ethylene glycol, 20%% glycerol; at −20 °C) such that sections of different sections of different age groups were processed at the same time.
Within papers, for instance, the practice of having separate batches of subs for each section - features, news, business and, more debatably, sport - has been gradually eroded.
A number of pieces will be shown from the first batch of movies, including sections of Poitras' own "Asylum," about Julian Assange.
Different case types were embedded in batches as they came from the tissue processor, then moved in batches to the microtomy stations where batches of slides were prepared, and the sections made.
