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With awareness of common contaminating species, careful collection of controls to cover different batches of sampling, extraction and PCR kits, and sequencing to monitor the content of these controls, it should be possible to effectively mitigate the impact of contaminants in microbiota studies.
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Two batches of samples were prepared.
This allowed the processing of large batches of samples, and their comfortable measurements with convenience.
Experiments were run in triplicate using three different batches of samples.
Three batches of samples were investigated by X-ray micro computed tomography and tested under fatigue.
For both batches of samples, two rounds of 2D classifications were performed to exclude noise and other bad particles.
The established method was applied successfully to analyze 24 batches of samples from six cultivars of C. morifolium.
RT-PCR analysis of marker gene expression was conducted in at least three batches of samples from separated ovarian cultures with similar results presented.
The experiments were repeated with three independent batches of samples.
Screening of suitable biodiesel feedstocks often involves sampling extremely large batches of samples.
The three batches of samples were therefore normalized (by RMA) independently of one another.
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