Exact(13)
The An batch used for the experimental procedures was assessed at different concentrations (10−100 μg/mL) for endotoxin contamination (left part of the figure).
Thus, SNPs, indels, and transpositions may pre-exist in the seed batch used for transformation.
For each tracer batch used for logD determination, the experiment was performed in duplicate.
A further 13 slides (7 from the batch used for the TB patients, and six from the batch used for the healthy subjects) were prepared using only buffer and secondary antibody, in order to help identify peptides giving a 'false positive' response, which were removed for further analysis (see below).
Total RNA from the same batch used for transcription profiling was treated with Dnase1(Invitrogen) and converted to cDNA with the High Capacity cDNA Archive Kit and random primers (Applied Biosystems, Foster City, CA), using 125 ng total RNA per 100 µl reaction for each sample.
These stocks constituted the challenge batch used for all experimental inoculations.
Similar(47)
Thus, the true release levels of batches used for shelf life determination are assumed to be representative of those of future lots.
The following effects were included in the analysis: WT-Li vs WT-untreated as the biological effect, colony (IMPA1 or SMIT1) and batch as random effects (batch effect relates to the overall differences between the experiment of the IMPA1 colony vs the SMIT1 colony in terms of the different colony background, the Affymetrix batches used for each of the colonies, the experimental dates, etc.).
Ultrasound-assisted extraction of V and Ni from dried mussel tissues using probe sonication allowed a fast solid liquid extraction thus facilitating sample preparation from large sample batches used for biomonitoring.
There were identical water and sediment batches used for the saturation step and for the actual test.
The PCR bead kit from Illustra was used in both the Linköping and Stockholm laboratories, with new batches used for each PCR amplification setup.
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