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In order to explore the aggregate specific rates of growth and donor oxidation, the batch tests were fitted to determine the "apparent" or extant specific growth rates and donor utilization rates.
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Batch tests were conducted in duplicate, in 1 L Erlenmeyer flasks (working volume: 0.7 L), fitted air-tightly with rubber septum, and adequately sealed using commercially available fix gels.
Batch tests were performed in a double-jacketed batch reactor.
Duplicate sequencing batch tests were performed.
Batch tests were carried out at least in triplicate.
Sex and batch were fitted as fixed effects in the model.
As in the previously described GWAS, sex and batch were fitted as fixed effects.
Within batches models were fitted in parallel.
Samples for batch test were collected in June and September.
Each batch test was carried out at least in triplicate.
Briefly, univariate mixed models of variance were fitted for each expression trait, using as fixed effects slaughter batch (7 levels), hybridization batch (2 levels) and spotting batch (2 levels).
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