The straws originated from 6 semen batches (quantification cycle [Cq] values 26.4 36.4) collected from 6 bulls (designated A C and E G) during August and September 2012 (1 ).
Heart-field size can vary somewhat both between batches of embryos and between embryos from the same batch of eggs so quantification of absolute heart size by area measurement does not allow statistically meaningful comparison between experiments.
It does not require RNA extraction and 96-well plates with serially diluted cells can be stored at − 80 °C for future quantification in batch analyses, making TILDA well-suited for longitudinal clinical studies.
Quantification of the "batch-based" reassemblies against the original single-BAC assemblies was done using Vmatch [ 35].
Stitching and batch processing of images, and H3P quantifications per surface area were performed as previously reported (Adler et al., 2014).
Triplicates of quality control samples at three concentrations (3.46 ng/ml, 36 ng/ml, and 375 ng/ml) for both artemether and dihydroartemisinin were analyzed within every batch to ensure precision and accuracy during quantification.
Quantification was possible in 5 batches of faecal samples (2.4 350 MPN g− 1 of Salmonella).
The system is proposed to be an easy and cost effective system for quantification of BALP as an alternative to batch wise wheat germ specific binding technique.
Thus, experiments, quantification, and comparison were conducted with each batch.
A representative batch of ZERA-GLA was chosen as a positive control and quantification standard by first quantifying against a commercial GLA from E. coli 12078-H08H (Sino Biological Inc. Beijing, China) using GelCode Blue G250 Coomassie stain (Thermo).
Prior to infection, high-titer lentiviral stocks were obtained by ultracentrifugation and the particle content of individual batches was determined by p24 antigen ELISA and RT-qPCR quantification of viral RNA [45].
