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The continuous culture was performed in a 1.5 L fermentor (Applikon, Netherlands) at a working volume of 1.2 L. The initial glycerol batch phase was performed in basal salts medium, which was inoculated from a 30 ml overnight YPD culture grown to an OD600 of ∼0.7.
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A linear methanol fed-batch phase was performed for 60 h (final biomass yield 115 g L−1; Hohenblum et al. 2004).
Phasing was performed on 54,787 shared SNPs across all 1,928 individuals from the 73 Asian and two non-Asian HapMap populations within the same batch runs, which did not consider the existence of different populations.
A fed-batch phase was then performed using 552 mL SWM (318.6 g/L glucose), fed over a period of 24 h, at a constant feed-rate.
In fed-batch processes, provision of extra nitrogen during the batch phase was useful to provide rapid biomass production, with lipid accumulating during the feeding phase.
Growth phase in batch was performed on 15 g/L glucose at pH 4.8 and 27 °C for 24 h.
Growth phase in batch was performed on 15 g L-1 glucose at pH 4.8 and 27°C for 24 h.
The highest value for the growth rates in the batch phase were between 0.38 and 0.19 1/h while in the fed-batch phase values below 0.052 1/h were computed.
A glycerol batch phase is followed by a transition phase and by a methanol induction phase [ 14].
Batch adjustment was performed in two batches.
No further batch correction was performed.
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