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Sufficient biomass is first generated in a batch phase.
The batch phase was on 40 g l−1 glucose, once the glucose was consumed, the constant feeding of glucose or ethanol was initiated.
The model-derived values for the growth rate show three distinct phases, reflecting the batch phase before 14 h and the fed-batch phase thereafter.
In this batch phase, no sampling or peptone addition was performed.
The initial cultivation terminated when all glycerol was consumed (about 14 h) at batch phase.
The batch phase took almost 2 h until the concentration of glucose and ethanol was depleted.
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The product yield increased from 0.075 to 0.104 g/g during the fed-batch phase.
During the initial part of the fed-batch phase, the specific biomass and CO2 productivity were identical to TMB4530.
This induction step is called the fed-batch phase.
In the second step, when initial lactose was depleted, a fed-batch phase was initiated.
No carbon source accumulation was observed during the whole fed-batch phase.
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