Growth and survival of Greenshell™ mussel (Perna canaliculus) larvae were identical when fed with C. calcitrans batch culture or continuous culture delivered directly from the harvest line.
The effect of soluble carbon sources in fed-batch or continuous cultures at the laboratory (2 L) and pilot (3 and 30 m) scales has already been reported [ 11, 12, 22, 23].
Adaptation, either by serial batch or continuous cell culture, has been successful for improving growth and fermentation of S. cerevisiae strains expressing different D-xylose isomerase genes [ 6, 14] as well as for cells expressing the reductase/dehydrogenase genes for D-xylose metabolism [ 7].
The chemostat cultivations, consisting of batch cell growth (batch) and continuous culture with glycerol or methanol feeding, were performed in a 2.0 L working volume bioreactor under computer-based process control.
Operational practices such as batch, fed batch, and continuous culture are described.
In particular Namjoshi et al. (Biotechnol. BioEng. (2002), accepted) have been able to predict the transition from batch and fed batch to continuous culture in hybridoma experiments (Biotechnol. BioEng. 67(1) (2000) 25).
The integration of the box model into radiative transport equation (RTE) is verified first and then utilized to predict the microalgae concentration evolutions in a batch and continuous culture, respectively, which are in a good agreement with the experimental data.
No H2 gas was detected in the culture headspace from both batch and continuous culture using gas chromatography suggesting that M. smegmatis did not produce detectable levels of H2 using the culture conditions employed in this study.
