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As such, an added benefit of having access to a long-term culture model that stably maintains a consistent phenotype and genotype over prolonged periods is that multiple studies or multiple repeat doses can be performed with a single batch of tissues or cells.
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Decay-corrected counts-per-minute data of the daily batch of tissue samples (≈100 for 2 compounds) were measured on a γ-counter LKB Wallac 1282 Compugamma within 2 h.
Each batch of tissue samples required a minimum run time of 3 days from tissue processing to result.
This result and all subsequent Western blot analyses presented have been confirmed on at least three batches of tissues.
Seven batches of tissue samples were subjected to 2-DE.
The statistical analysis of the volumes (% volume) of these spots from seven batches of tissue samples is shown in Figure 2B.
To ensure reliability and reproducibility, seven batches of tissue samples were subjected to proteomic analysis using ImageMaster 2D Platinum Version 5.0 software and fourteen gels were generated.
Leaves from five virus-infected grapevines along with their respective controls were used separately and pigments estimated by two independent times using separate batches of tissue.
Three, and two, recipients received grafts treated with LIF-nano, or empty-nano, respectively: each batch of donor tissue was equally divided between each treatment group to ensure equivalence.
Advanced protocols such as the batch-isolation of tissue specific chromatin for immunoprecipitation (BiTS-ChIP) have been successfully used to isolate chromatin even from fixed nuclei thereby allowing a direct view at the chromatin at the time of tissue isolation (Bonn et al, 2012a; Bonn et al, 2012b).
In each batch of CXCR4 staining, tissues from breast cancer served as positive controls, whereas omission of primary antibody was used for negative control.
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