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In order to obtain a gauge of the mixing error present in our experiment and to ascertain that our results were not the cause of an unexpected bias, we made a new batch of solutions for 500, 400, 300, and 200 that were independent of both the solutions used for the data set in Table 1 and each other (they were not created through serial dilution).
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If your shoes are covered in mud, you might need a second batch of solution for the second shoe.
Quantitative comparisons were carried out on sections processed simultaneously using same batches of solutions.
Serial sections from control (WT) and both tg mice (PS1 and PS1/APP) were processed in parallel for light microscopy immunostaining using the same batches of solutions to minimize variability in immunohistochemical labeling conditions.
Serial sections from control and lithium-treated transgenic mice (n = 6 per group) were processed in parallel for immunostaining using the same batches of solutions to minimize variability in the immunohistochemical labeling conditions.
In a typical batch process with a liquid solution, a batch of the solution is mixed with a portion of the adsorbent for a determined length of time, after which the two phases are separated.
For each batch of prepared solutions, 5 bottles were randomly selected, transferred to a collaborating pharmaceutical laboratory at Eskayef Bangladesh Limited, and analyzed for final CHX concentration.
The resin was exposed to the deprotection solution 20% piperidine in DMF for 30 s under microwave irradiation at 75 °C (35 W microwave power), the solution is removed, a fresh batch of deprotection solution and the step repeated for 3 min. The resin was then treated with DMF (4 × 2 min) then treated with 3% hydrazine in DMF (4 × 15 min) to remove the ivDde group.
This step was performed once, each time a new batch of [18F]fluoride solution was connected, before automated aliquoting.
In regard to the development and production of radiotracers, a tool that allows for automated aliquoting of user-specified amounts from a batch of [18F]fluoride solution will eliminate the need for radiation workers to manually draw radioactivity.
To dispense in this fashion, a custom-designed radiation detector (see "Radiation detection technique" section) was used to determine the reference radioactivity concentration (i.e., mCi μL−1) during a one-time calibration step per batch of radioactive solution.
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