In order to obtain a gauge of the mixing error present in our experiment and to ascertain that our results were not the cause of an unexpected bias, we made a new batch of solutions for 500, 400, 300, and 200 that were independent of both the solutions used for the data set in Table 1 and each other (they were not created through serial dilution).
In a typical batch process with a liquid solution, a batch of the solution is mixed with a portion of the adsorbent for a determined length of time, after which the two phases are separated.
The resin was exposed to the deprotection solution 20% piperidine in DMF for 30 s under microwave irradiation at 75 °C (35 W microwave power), the solution is removed, a fresh batch of deprotection solution and the step repeated for 3 min. The resin was then treated with DMF (4 × 2 min) then treated with 3% hydrazine in DMF (4 × 15 min) to remove the ivDde group.
Quantitative comparisons were carried out on sections processed simultaneously using same batches of solutions.
Serial sections from control (WT) and both tg mice (PS1 and PS1/APP) were processed in parallel for light microscopy immunostaining using the same batches of solutions to minimize variability in immunohistochemical labeling conditions.
Serial sections from control and lithium-treated transgenic mice (n = 6 per group) were processed in parallel for immunostaining using the same batches of solutions to minimize variability in the immunohistochemical labeling conditions.
This step was performed once, each time a new batch of [18F]fluoride solution was connected, before automated aliquoting.
In regard to the development and production of radiotracers, a tool that allows for automated aliquoting of user-specified amounts from a batch of [18F]fluoride solution will eliminate the need for radiation workers to manually draw radioactivity.
To dispense in this fashion, a custom-designed radiation detector (see "Radiation detection technique" section) was used to determine the reference radioactivity concentration (i.e., mCi μL−1) during a one-time calibration step per batch of radioactive solution.
Mix up a large batch of bubble solution with the following ingredients: 1 cup liquid soap.
