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In every batch of samples analyzed for values of Rr2, tetracycline's must be at least 0.8278, while the sulphonamides' Rr2 > 0.98.
The analyses were calibrated using the reference material BHVO-1, and Mud Tank Carbonatite apatite, and international rock standard BCR-2 were used as check standards with each batch of samples analyzed.
For the entire batch of samples analyzed for cases and controls, the CV was 8.8%.
Negative and positive controls were included in every batch of samples analyzed.
The bias between the nominal As level in the standard and that calculated was determined for each As form in each batch of samples analyzed.
Every batch of samples analyzed included a calibration check (1 and 10 ppb), a calibration blank (0 ppb), 10 field samples, a control sample [a standard reference material (SRM) or LC for drinking water, and a method control (MC) or certified reference material (CRM) for food and hair], and an independent check standard (10 ppb).
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The precision was ≤ 4% across all batches of samples analyzed, and the bias was ≤ 6%.
Three working standards calibrated to VSMOW and standard reference waters supplied by the International Atomic Energy Agency IAEAA) were analyzed in duplicate with each batch of water samples analyzed.
These quality control samples were then included with each batch of experimental samples analyzed.
Quality control (n = 3) and method blank (n = 3) samples were included in every batch of serum samples analyzed (n = 24).
To assess method performance at the primary laboratory, quality control samples in the form of two control serum blanks, two lab control spikes in control serum, and two sample duplicates were included with each batch of 90 samples analyzed.
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