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Parallel samples were processed using the same batch of reagents to minimize overall sample-to-sample variations.
All samples were tested with the same batch of reagents.
Traditionally, industrial enzymes are added into a batch of reagents in reactors during chemical processing.
All gels were run under identical conditions with the same batch of reagents and densitometry results standardised between gels.
10 Thus, we retested aliquots of serum from all patients using an unaffected batch of reagents and repeated all statistical analyses, which are presented here.
Equal amounts of mRNA were included per reaction, and all samples were tested in a single experiment using the same batch of reagents.
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All of the individual samples were stored at −80°C until analysis when they were assayed using one batch of reagent.
Calibration was performed with each new batch of reagent or every 2 days, whichever was sooner, according to the manufacturer's instructions.
One study [ 26] reported a LCMM patient missed by Freelite; however the sample was correctly identified when reanalysed using the same batch of reagent, indicating a previous analytical error (personal communication).
Single batches of reagents and a single lot of Affymetrix GeneChips were used for all samples within a cohort.
This procedure can be easily and routinely applied to batches of reagents, which can then be stored at −20°C for later use.
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