Sentence examples for batch of primers from inspiring English sources

Exact optimality criteria used for the selection of each batch of primers is available from the authors.

As part of our quality control, every new batch of primers is tested against a set of 95 samples to confirm well-balanced amplification, i.e. no second allele detected with less than 20% of the reads of the dominant allele.

Strengthening of these areas consisted of first preparing a batch of primer mixed with sand and applying grout mixture to the three areas of strengthening.

First, we carried out RT-PCR experiments using different batches of primers and several RNA extracts of zebrafish embryos at various developmental stages, as well as adult organs.

Optimization includes temperature gradient selection of the annealing temperature, random population screening for common variants, and batch preparation of primer plates with robotically deposited and dried primer pairs.

Primers were designed to amplify both in a typical inward direction, and in a reverse orientation between gene copies using the Primer3 software in batch mode (table of primers available on request).

To this end batches of PCR primer were designed by the Primer3 software driven by an in-house Perl script.

Negative control without cDNA template and control of genomic DNA contamination in RNA samples were performed for each pair of primers and each batch of RNA extracted.

Three independent PCR reactions with two different sets of primers and three DNA batches were used to monitor the presence of genes in the strain collection.

In 12 cases inwardly directed gene specific primers produced a PCR product from this batch of cDNA, but when a trans-spliced leader primer was used as the forward primer no product was seen.

Generally, two sets of primers were used for each gene on three batches of DNA (See Additional file 4: Table A4 for the sequences of primers).