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Plates 31 to 39 focus on atrocities and were produced on the same batch of plates as the famine group.
A single batch of plates was prepared and kept frozen.
The laboratory-adapted strain 3D7, tested 3× on the same batch of plates, was used as reference.
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Few of the plates or drawings are dated; instead, their chronology has been established by identifying specific incidents to which the plates refer, and the different batches of plates used, which allow sequential groups to be divined.
The intraplate and interplate CV for each cell line was calculated in at least three separate experiments over three different batches of plates.
P. falciparum clones 3D7 (1 Pfmdr copy) and W2 (3 Pfmdr1 copies) were used as controls for the determination of the Pfmdr1 copy number and for the validation of the batches of plates used in the susceptibility tests.
The interplate CV gave values of 17.88% for U-87 MG, 11.21% for KNS42 and 10.75% for LICR-LON-HN4 (n = 3, tested over three different batches of plates).
Meanwhile, a batch of 30 plates including duplicates from 15 source plates of 384-well siRNA library were thawed at room temperature, centrifuged, and cleaned with RNAse-reducing solution (Life Technologies).
Chemical library plates were prepared and assayed as one batch of 15 plates.
Production was delayed by quality problems with the initial batch of armor plates from Poldi and delivery of the pre-production series did not occur until 23 April 1934.
Cells from a sector were harvested and enumerated by CFU assay after 24 h, and a second batch of inoculated plates was enumerated after 48 h (Figure 6C).
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