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One of the infected plants from each lineage was randomly chosen to be the source of viral particles for infecting the next batch of plants.
In another batch of plants, the first leaf was inoculated with C. lagenarium.
Subsequently, material from the same batch of plants was used for dual-color microarray hybridizations of stressed and FN samples.
When the second batch of plants grew to the booting stage in the autumn, no obvious lesion mimic was observed in these plants.
Two of the mature pollen samples were the ones used in the miRCURY array, while the third one was isolated from a different batch of plants.
A second batch of plants were grown under the same conditions for 6 weeks, then transferred to the field and measured 5 days later.
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For optimal efficacy, every single batch of plant extracts is ensured to the exact percentage of the standard markers.
The quantitative PCR experiments were repeated at least three times for a cDNA prepared from three batches of plants.
All experiments were repeated three times for cDNA prepared from three batches of plants.
Therefore, we sought to randomize such influences by performing most experiments in triplicates from three spatially and temporally separated batches of plants, with all conditions represented in each batch.
Average weight and number of mature seeds was determined in 4 independent batches of plants.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com