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Sugar uptake in non-injected oocytes from the same batch of oocytes was used as control.
For each batch of oocytes, individual GIRK currents from the isoform-expressing group were normalized as a percentage of the average current of the vector control group.
For each batch of oocytes, individual GIRK currents in isoform injected oocytes were expressed as a percentage of average current in vector control oocytes.
Five to seven oocytes per construct were analyzed individually for each batch of oocytes.
For each batch of oocytes, all mutations were injected to enable direct comparison of their effects.
The Ps of oocytes for each of the three cryoprotectants was measured using the same batch of oocytes.
Similar(45)
All experiments were performed on minimum two batches of oocytes.
Measurements were repeated for at least 5 different batches of oocytes.
In early experiments, there was considerable variation in the intron-integration efficiency between different batches of oocytes (insertion frequencies 0.002 7%).
Previous WT Kv4.3 data [9], [10], [16], [25] used for comparison were acquired in each study from a minimum of three different batches of oocytes.
Staining and imaging was repeated with two different batches of oocytes for a total of 75 oocytes stained for each strain (50 oocytes/Sta. Cruz antibody and 25 oocytes/Alexis antibody).
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