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If any sample shows contamination in repeated samples, the whole batch of medium should be discarded.
Then, an aliquot of cells was transferred to a new batch of medium.
This second batch of medium was then collected and the cells discarded.
Every 24 hours, small aliquots of cells were transferred to a fresh batch of medium, and the cells that were not transferred were discarded.
The modified PEHPS medium offers the advantage of being inexpensive and very stable during refrigeration and freezing, so that a large number of experiments can be performed with the same batch of medium for more than 6 months without affecting yields [ 9].
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Bar products from a commercial batch of medium-carbon chrome-molybdenum steel, JIS SCM440, were processed in three different ways in order to produce the specimens of different microstructures but of an almost identical hardness.
The same batches of medium constituents were used in all experiments to minimize experimental error.
The explanation is likely related to the different conditions of the experiments, e.g. different batches of the MCF-7 cell line and different batches of medium.
Most dusky salamanders (Desmognathus) and Pacific giant salamanders (Dicamptodon) lay smaller batches of medium-sized eggs in a concealed site in flowing water, and these are usually guarded by an adult, normally the female.
Then CLEA-protease was resuspended in a fresh batch of reaction medium for 2 days.
The endotoxin concentration in a batch of unconditioned medium without albumin was <0.5 and 1.6 pg/mL (duplicate).
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