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In order to avoid cross contaminations, extraction and pre and post-amplification procedures were performed in different rooms and we used negative controls for each batch of extraction (30 samples), and in all amplifications.
One HPV-negative sample was included in each batch of extraction to exclude any contamination.
An HPV negative sample was included in each batch of extraction to exclude any contamination during this phase.
In each batch of extraction, a negative control that consisted of 200 μL of PBS was used to monitor for cross-contamination.
With each batch of extraction, a negative control consisting of 200 μl of PBS was used to monitor for cross-contamination.
To prevent and monitor the contamination of the samples during the laboratory processes, pre-PCR and post-PCR activities were carried out in different laboratories and negative controls were included in each batch of extraction and amplification [ 52, 53].
Similar(54)
At least one blank was processed with each batch of extractions.
For each batch of extractions, a negative extraction control containing all reagents minus sputum, was performed, as well as an extraction positive control with P. aeruginosa.
One set of double distilled water was included in each batch of DNA extraction to check cross-contamination of DNA during DNA extraction.
The spectrum of the extracellular pigment showed that the yellow pigment was transported more during the first batch of the extraction because the peak wavelength of the spectrum was approximately 380 nm (Fig. 3A, e1).
A positive sample and a negative control, i.e. TE buffer in place of sample were run in each batch of DNA extraction.
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