Either for Cry8Ka5 or for Cry1Ac, the different batches of expression and purification presented relative purity which ranged from 75% up to 95%.
Other possible workflows that could be implemented in Taverna include cross-species comparisons of pathway information, or batch visualization of expression data.
With lysate from a better batch of protein expression there is a possibility that all of the sequences may demonstrate higher binding reactivity to tumor than normal sera.
The process during which batches of compounds, expression libraries or RNA-interference libraries are tested for a defined activity on a certain target.
2. Chen C, Grennan K, Badner J, Zhang D, Gershon E, Jin L, Liu C: Removing batch effects in analysis of expression microarray data: an evaluation of six batch adjustment methods.
Cells were changed due to problems with the old batch losing expression of CGRP receptors over passages, as has been documented previously (Choksi et al., 2002).
Whereas expression of EWS-FLI-1 was tolerated in all of the cell batches tested, expression of EWS-ERG was restricted to a fraction of the batches while stable expression of FUS-ERG could not be achieved.
A batch of breast cancer-related expression data was extracted from the GEO database [ 38] on April 7, 2011 and expression profiles in those data files were clustered based on their similarity with each other (Box E).
With experimental variation from batch to batch, the expression yields of all three hits were 5- to 10-fold higher than that of the WT.
fRMA is designed to normalize large amount of heterogeneous microarray samples to reduce the effect of batch on gene expression estimates.
