Sentence examples for batch of cells were from inspiring English sources
Every four days (approximately four days before the peak of infection was reached) virus was harvested and a new batch of cells were infected.
The eGFP+ MHC class I- cells as well as untransduced hMSCs from the same batch of cells were then exposed to the vesicular stomatitis virus G protein-pseudotyped self-inactivating lentiviral vector LV.C-EF1a.cyt-bGal [70], encoding Escherichia coli β-gal.
Another batch of cells were cultured in the presence of brefeldin A (10 μg/ml, Sigma) in addition to the culture conditions above.
Samples for each independently plated batch of cells were run in triplicate and average intensity readings were normalized to their respective loading control expression or total eIF2α expression (for p-eIF2α quantification).
Then for 5 days, every 24 h a batch of cells were stained with 20 μl sterile MTT dye (5 mg/ml; Sigma, USA) at 37°C for 4 h, then culture medium was removed and 150 μl of DMSO was added and thoroughly mixed in for 10 min. Spectrometric absorbance at 490 nm was measured by using a microplate reader.
They now plan to try growing ovary stem cells from common frogs and, when they find a technique that works, remove some of the yellow-legged frog cells from the cryogenic chamber and test it on them.Although this particular batch of cells is unlikely to be used for assisted reproduction because of the donor's heritable mutation, it will help scientists work the process out.
In addition another batch of cells was produced in house, but without an anti-reflection coating.
When a batch of cells was connected and located near the pipette, the pipette was also successfully identified as shown in Figure 12d.
As the structural element and threshold value are the key parameters for the successful identification of cells, a batch of cells was tested independently, and a certain range of size of structural element and threshold values were applied.
